Green fluorescent protein-based biosensor to detect and quantify stress responses induced by DNA-degrading colicins.
نویسندگان
چکیده
Here we report the development of a whole-cell biosensor to detect and quantify the induction of the SOS response activated by DNA-degrading colicins. This biosensor utilizes the SOS-responsive cda promoter to regulate the expression of green fluorescent protein. The biosensor assay revealed induction of stress for all DNA-degrading reference colicins (E2, E7, and E8).
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 77 18 شماره
صفحات -
تاریخ انتشار 2011